LSIT platform
Cellytics NK – how it works
LSIT
Lens-free Shadow Imaging Technology
Cellytics™
Simple structure
01
LED & micro-pinhole
02
Disposable cell chip
03
CMOS image sensor
Digital In-line Hologram
DIH (cell shadow) capability
01
Single cell analysis
02
External & Internal property
03
Shadow library (AI & Big data)
Shadow Parameters
Cellular information with
CMV, PPD, MMD, MCD, WCX, WCN, SSM, SMD, etc.
[Lab Chip 2009, Anal. Chem. 2010, Biosens. Bioelectron. 2012, 2015, 2017, 2024]
ASC
Activation Stimulator Cocktail
Optimized rapid cell activator ASC
Activation of target cell
Optimized to Cellvtics®
Validation of pharmaceutical candidates
ASC-NK (patent filed)Activator of NK cells
ASC-TBT cell activator for LTBI diagnosis
ASC-MQMacrophage activator
ASC-NeutrophilNeutrophil activator
Development of other specific immune cell activators
‘Count’ and ‘Activity’ at ONE TIME!!
Overcoming limits on Assay Standardization and Accessibility
ELISA | Cellytics® | FACS | |
|---|---|---|---|
Complexity | Intermediate | Low | High |
Analyte | Cytokine (e.g. IFNy) | Cell | Fluorescence labeled Cell |
Method | ELISA | Digital In-line holography
(Cell imaging without labeling) | High |
TAT | 2~3 days | < 2 Hours | ~3 Hours |
Cost | $40 | $20 | $100 |
Reliability | Intermediate | High (single cell level) | High (single cell level) |
+ Count
Applications
Cellular Functional Assays
Hematology analysis
01
Cell counting
HLA crossmatch
02
Cell Viability
NK cell activity test
03
Cell therapeutics potency assay
Latent Tuberculosis Infection Detection
04
Water Quality Monitoring
Medical
Research